![]() ![]() The Pierce Reversible Protein Stain Kit for PVDF Membranes has excellent avidity for a broad range of proteins, resulting in low protein-to-protein variability and enabling most proteins to be detected at very low levels (25 to 50ng per band). Subsequent western blot detection is unaffected because the stain does not alter the protein and is completely removed. The stain can be easily reversed in less than 15 minutes. The staining protocol is simple, quick and results in turquoise-blue bands that do not fade and are easily photographed for future reference. The lower limit of detection with this method is 25 to 50 ng per band (at least five times more sensitive than traditional Ponceau S staining). The kit is a superior alternative to Ponceau S stains for evaluating the efficiency of protein transfer following SDS-PAGE and before immunoblotting. This kit for membrane staining uses a nondestructive, reversible, reliable and sensitive method to stain and detect proteins on PVDF membranes. Stable-components are stable at room temperature, saving refrigerator space and eliminating equilibration steps.Rapid-stain in less than 30 minutes completely erase and reverse staining in less than 15 minutes. ![]() Specific-detects only protein does not bind or interact with other electrophoresis or sample components.Sensitive-high-avidity, total-protein stain lower limit of detection equals 25 to 50 ng per band.Better than Ponceau S-more sensitive, easier to document, permanent until reversed (does not fade).The Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes is a rapid and sensitive alternative to Ponceau S stain for protein detection on PVDF membrane after transfer from polyacrylamide gels.įeatures of the Reversible Protein Stain Kit for PVDF Membranes:
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